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Study on the isolation , identification and insecticidal activity of endophyte from Achnatherum inebrians
醉马草内生菌的分离、鉴定及杀虫研究

Keywords: Keyword: Achnatherum inebrians,endophyte,Streptomyces rochei,Claviceps purpurea,cotton
关键词:醉马草
,内生菌,娄彻氏链霉菌,麦角菌,棉花

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Abstract:

ObjectiveTo study endophyte species of Achnatherum inebrians and to screen strains with insecticidal activity against cotton insect.MethodsWe isolated endophytic from roots,stems,leaves and seeds of health A.inebrians by grinding separation method and identified by a dual approach of morphological and physiological observation and 16S rDNA gene(for bacteria) and ITS sequence(for fungi) based molecular identification.Then,those endophytes were inoculated into liquid media for fermentation and the crude extra...

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