Assessment of genetic diversity in Fusarium oxysporum f. sp. niveum by RAPD, ISSR and AFLP analysis
基于RAPD、ISSR和AFLP对西瓜枯萎病菌遗传多样性的评价

Fifty isolates of Fusarium oxysporum f. sp. niveum were analyzed with random amplified polymorphic DNA (RAPD), interal simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) techniques. The results showed that each 21 primers of RAPD, ISSR and AFLP generated 113, 134 and 389 polymorphic bands respectively. These three molecular markers had consistent genetic coefficient of similarity and revealed the genetic diversity of isolates in Fusarium oxysporum f. sp. niveum. The cluster results produced by the three techniques were different. For RAPD groups, the relation with both physiological races and geographic regions of isolates were all inconspicuous. The AFLP and ISSR groups were generally associated with the physiological races, whereas there was no obvious relationship with geographic regions of isolates.