TRANSMISSION,SEROLOGY AND PCR ANALYSIS OF TOMATO LEAF CURL VIRUS
番茄曲叶病及其血清学和PCR测定

Tomato leaf curl virus (TomLCV) disease was found in the suburb of Nanning city of Guangxi in the autumn of 1991 - 1992. It showed the symptoms of stunting, leaf curling, cup or ear-like enation on the back of leaves, dark green vein. Test indicated that it could be transmitted by grafting and whiteflies (Bemi-sia tabaci). The infected tabacco and tomato by TomLCV induced the same leaf curl symptom as that by tobacco leaf curl virus (TLCV), based on this result, the pathogen of tomato leaf curl disease was primarily refered as TLCV. Further studies showed that both TLCV and TomLCV infected leaf extract reacted positively to MAb-SCR18 of African cassava mosaic virus (ACMV). Both virus could produce about 500bp DNA fragments by PCR using the whitefly-transmitted geminivirus common primers. Restriction enzyme analysis (using Alul, Sau3A and TaqI respectively) of these two viruses PCR products produced the same results. All the above results further confirmed that the pathogenic virus of tomato leaf curl disease has high homology with TLCV.