PURIFICATION AND CHARACTERIZATION OF TWO TYPES OF POLYGALACTURONASE
两种类型聚半乳糖醛酸酶的提纯及性质

Two types of polygalacturonases (PG1 and PG2) were separated and purified by DEAE-Sephadex A50 and Sephadex G100 from crude pectinase produced from A. niger AS 3.3883. Some properties and structures were investigated with purified enzyme of PGl and PG2. The results indicate that the optimum conditions of their activity, kinetic parameters, molecular weight, composition of amino acids and the effects of some metal ions on activity of the enzymes are markedly different from each other, while the molar activity and the conformations of the two types, of PG are similar.