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微生物学报 2007
The investigation of Metarhizium anisopliae in forest soil in Fujian and Jiangxi provinces and pathogenicity against Monochamus alternatus
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Abstract:
Monochamus alternatus is the main vector of pinewood nematode, a critical plant quarantine organism. Three hundred and thirty soil samples were collected from 110 forest fields (of which 88 were pine wood fields) distributed over 40 counties in Fujian and Jiangxi province from August 2005 to September 2006. Metarhizium anisopliae was detected in 26 soil samples from 21 forest fields (of which 16 were pine wood fields) by selective medium, which accounted for 7.9% of the samples and 19.1% of the fields. Colony Forming Units (CFU) of M. anisopliae in the soils ranged from 500 to 72500CFU/g, indicating a wide distribution of this fungus. The pathogenicity of 9 well sporulating isolates against M. alternatus was evaluated by using adult-conidial attaching method and larva dip method. MaYTTR-03 and MaYTTR-04 have showed high virulence to M. alternatus and the mortality in 21d after inoculation was 80.0% and 76.0% for larvae and 83.4% and 88.9% for adults respectively at inoculation of 1.2×106 ± 2.0×105 and 1.5×106 ± 5.0×105 conidia/individual, indicating their high potential for management of M. alternatus.
