DETERMINATION AND STUDY OF DEOXYNIVALENOL (DON) IN WHEAT USING INDIRECT COMPETITIVE ENZYME LINKED IMMUNOSORBENT ASSAY BASED ON MONOCLONAL ANTIBODY
小麦中脱氧雪腐镰刀菌烯醇酶联免疫吸附测定方法的研究

A hybridoma cell line secreting monoclonal antibody againtst deoxynivalenol (DON) was established by fusion of spleen cells with myeloma. The antibody was designated 3D7 with the subtype IgG1. Meanwhile, indirect competitive enzyme-linked immunosorbent assay for determination of DON in wheat was developed with this antibody. The linear range for detection of DON was 5-1 000 ng/ml and the minimum detected concentration was 5 ng/ml. The mean recovery rates and CV% from sample spiked with 5-1 000 ng/ml of DON were 96. 6-107. 3% and 5. 1-14. 2%. respectively. Analysis of 10 samples for DON showed positive results, and the concentration ranged from 56. 4-1002. 0 ppb.