Novel Recombinant Escherichia coli Producing Ethanol from Glucose and Xylose
利用木糖和葡萄糖合成乙醇的新型重组大肠杆菌的研究

Genes pdc and adhB encoding essential ethanologenic enzymes in Zymomonas mobilis were amplified by using PCR technique. Recombinant plasmids pKK-PA, pEtac-PA were constructed in which genes pdc and adhB were placed under the control of tac promoter, respectively. Preliminary ethanol fermentation using the E. coli JM109 and its recombinants was carried out. The results indicated that introduction of adhB alone could not widen ethanologenic pathway in E. coli JM109.Introduction of pdc converted carbon flux to ethanologenic direction which was presumed to result from combined activities of pyruvate decarbonase from Z. mobilis and the native E. coli alcohol dehydrogenase. Introducing both pdc and adhB, ethanologenic pathway was successfully constructed in E. coli.