Cloning of Movement Protein cDNA of Tobacco Mosaic Virus and Its Expression in Escherichia coli
烟草花叶病毒运动蛋白cDNA的克隆及融合蛋白的表达

The movement protein gene was obtained by RT-PCR from the total RNA of tobacco mosaic virus and cloned to pMD-18T vector. Its length was 807 bp, compared with TMV and ToMV in GenBank, the identities of neucleotides and amino acids were 98.5%, 80.9% and 99.1%,80.0% respectively. The target fragment was subcloned to pET-30a, an expression vector. The fusion protein was expressed in the stain JM109 of Escherichia coli. The highest amount of expression time of the fusion protein which was solvent after sonication, was 9h after inducing.