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OALib Journal期刊
ISSN: 2333-9721
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Cloning and restriction analysis of saw1--a key gene for differentiation of Streptomyces ansochromogenes]
圈卷产色链霉菌分化关键基因——saw1的克隆及酶切分析

Keywords: Streptbmyces ansochromogenes,Differentiation,Gene cloning
Streptbmyces
,ansochromogenes,Differentiation,Gene,cloning

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Abstract:

saw1--a essential gene for sporulation of Streptomyces ansochromogenes was cloned, 7 kb of DNA fragment in Streptomyces plasmid pIJ702 could recover the sporulation of saw1-deficient strain W51 which could not form spore with white phenotype of aerial hyphae. The recombinant plasmid was deleted by some restriction enzymes, the result showed that 2.5 kb DNA fragment in pIJ702 still has function to recover sporulation of W51. The sequencing and expression of saw1 gene is carrying out at present.

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