CONSERVATION OF FRANKIA CULTURE
Frankia菌种保藏

Four conservation protocols of Frankia cultures were tested. Measured cell survival, morphology and nitrogen fixation activities of Frankia for each conservation protocol. Most sporangia and hypha were broken after storage in simple nitrogen-free liquid medium for 6 years. However, cultures stored in N-containing simple liquid medium for same time remained partially intact. Cultures stored in lyophilized milk for 3. 5 years or in sterile sand for 8 years were mostly intact. All the cultures mentioned above grew well and showed nitrogenase activity as well as typical morphological characteristic after an adaption period. Compared with other protocols, simple nitrogen-free liquid medium protocol were shown better in the item of cell growth, infectiveness and effectiveness.