PRODUCTION OF VITAMIN C PRECURSOR 2-KETO-L-GULONIC ACID FROM D-SORBITOL BY MIXED CULTURE OF MICROORGANISMS
微生物混合培养从D山梨醇产生维生素C前体——2-酮基-L-古龙酸

Gluconobacter oxydans SCB329 only produce a little amount of 2-Keto-L-gulonic acid(2-KLG)from D-Sorbitol when growing alone;while Gluconobacter sp. SCB110 can transform D-Sorbitol to L-Sorbose and can not produce 2-KLG.2-Keto-L-gulonic acid, the precursor of L-Ascorbic acid (Vitamin C) synthesis, was prepared directly with a high efficiency from D-Sorbitol by mixed culture of microorganism, which comprised Gluconobacter sp.SCB110 and Gluconobacter oxydans SCB329.The fermentation product from the mixed culture broth in the D-Sorbotol-containing medium was identified as 2-Keto-L-gulonic acid by HPLC, elementary analysis and infra-red adsorption spectrum.