Microsatellite Motif (TATG)n in Lentinula edode
微卫星(TATG)n基序在香菇菌种中的验证

The first microsatellite motif (TATG) n in cultivated edible fungi from Agaricus bisporus was tested in Lentinula edode strains. A 16 mer oligonucleotide (TATG) 4 was synthesized and used as primer in PCR with the DNA of 13 Lentinula edode strains as template.25 DNA fragments were amplified, and the strains were identified according to their polymorphs. Bands of No.10 strain were obtained from 1 5% agarose gel, sequenced, indicating that (TATG) 4 repeats are present in Lentinula edode . The strains were also tested by RAPD technique and 102 DNA fragments were amplified by 7 random primers. The cluster analysis results proved the results by Microsatellite. The variability found within closely related strains indicates that such microsatellites are useful in studying genetic variability in wild and cultivated Lentinula edode .