CONSTRUCTION OF MUCOSAL VACCINE DERIVED FROM HBV SURFACE ANTIGEN EPITOPE A AND THE ASSAY OF ITS IMMUNOGENICITY
乙肝表面抗原a表位粘膜疫苗的构建及免疫原性检测

The fusion gene of HBV(adr) surface antigen epitope and B subunit of Cholera toxin was constructed and expressed successfully in E.coli at high yield. After denaturation\|renaturation process, SDS\|PAGE analysis showed that most of the renatured product reassociated in a pentameric form which was the same as natural CTB. Western blot analysis indicated that the immunogenicity of HBsAg antigen epitope was conserved. Moreover, ELISA analysis of the sera of orally, intranasally and intraperitoneally immunized mice showed that the circulating IgG antibodies to HBsAg were developed. The results may be helpful for constructing the novel mucosal vaccine with high efficacy.