SCREENING AND CHARACTERISTICS OF MUTANTS OF E. COLI RESISTANT TO ACETATE INHIBITION
大肠杆菌乙酸代谢突变株的选育和特性研究

Acetate is accumulated in the aerobic high cell density culture of Escherichia coli ,Which seriously inhibits cell growth and expression of recombinant proteins.To alleviate acetate inhibition,mutants of E.coli JM101 were generated by 60 Co radiation and then enriched in continuous culture with acetate as the selective pressure.One of the mutants isolated,JL3,showed obvious increased tolerance toward acetate and maintained phenotypic stability on slant without acetate.In MA medium containing 10g/L of sodium acetate,the specific growth rate and the glucose consumption rate of JL3 were higher than those of JM101.