NUTRITIONAL REGULATION OF SYNTHESIS OF LIGNIN PEROXIDASE BY PHANEROCHATE CHRYSOSPORIUM ME-446
黄孢原毛平革菌合成木素过氧化物酶的营养调控

The effects of nutritional conditions on synthesis of lignin peroxidase and its isoenzymes by Phanerochate chrysosporium ME-446 were studied. Under the optimal culture conditions, the activity of 1 500U/L was obtained. Of the five isoenzymes separated with high performance liquid chromatergraphy,P2 content is the highest. Low carbon and high nitrogen medium is most suitable for synthesis of the enzyme. Lower contents of nitrogen or KH2PC)4 make decrease of all iosenzymes, while changing concentrations of MgSO4 or CaCl2 has no effects on P2 component. Tween80 mainly increases permeability of cell membrane. Veratrylalcohol induces synthesis of each of lignin peroxidase isoenzymes. There exist complicated mutual functions between nutritional factors in culture on the enzyme synthesis.