The Kinetic Characteristics of Mycelium Growth of Medicinal Mushroom Ganoderma sinense in Liquid-submerged Cultivation
药用蕈菌紫芝液体培养的生长特征分析

The study was carried out to investigate the kinetic characteristics of Ganoderma sinense mycelium growth in liquid-submerged cultivation, using a air-lift bioreactor ALR/ff specially designed for the cultivation of mushrooms' suspending mycelia cultures. Mycelia of G. sinense grew in the range of 25 degrees C-35 degrees C, and at the different temperature in which the specific growth rate showed by the mycelia much differed. The specific growth rates with the maximum of 0.0444(h-1) at 1.64 vvm of aeration in the cultivation were more than at 0.93 vvm, also more than at 2.50 vvm. In comparison with the less glucose concentration, the culture of 2.80 g glucose/100 mL in the medium had a shorter lag-period, and the more concentration of glucose favoured mycelia growth in the latter cultivation course with the denser pellets of mycelia. Then the culture of the less concentration of glucose in the medium had the higher conversion-rates of glucose to G. sinense biomass all along the cultivation course, than of the more concentration of glucose. Futhermore, in the continuous cultivations with growth-limiting substrate of carbon source the mycelia displaying different growing activities, demonstrated the varied forms of mycelium-gathering. In that cases the relationships among mycelia biomass, growth-limiting substrate and productivity of mycelium biomass obviously differed from the ones in the case of bacteria. Also in the range of dilution rates 0.010-0.220 (h-1) the growing of G. sinense mycelia followed the function of Contois equation.