THE ENVELOPE FORMATION OF AcMNPV AND THE LOCATION OF ENVELOPE PROTEIN gp64 IN HOST CELL
AcMNPV的囊膜形成与囊膜蛋白gp64在细胞内的定位

Electron Microscopic study on the recombinant AcMNPV infected Sf9 cell showed that the virus has at least two forms of envelope formahonf one is budding from plasma membrane and the other is enveloping in the nucleus by membrane structure. An additional possible pathway is budding from nuclear membame. The envelope formation of AcMNPV in Sac cell line and its relationship with the envelope protein gP64 were inveshgated by using immunofluorescence techniques. The results showed gP64 was mainly located on the plasma membrane and nuclear membr'ane of infected cell. Whereas, there is no gp64 on the envelope of OV virus prides in the nuclei. ms pattem cause the differences in envelope composition betWeen the virus Particles that budding from Plasma membrane and nuclear memb~e and those that enveloping in the nucleus.