Studies on Structure and Function of nisZ Promoter
nisZ启动子结构与功能的研究

Glucuronidase( gus A) gene was used as reporter gene for analyzing nis Z promoter. Two promoter regions(promoter1 and promoter2) upstream of nis Z code region were deleted respectively by site directed mutagenesis. Only promoter2, the promoter region near the code region, was involved in the induction and expression of nis Z promoter. nis Z promoter induction level was reduced when 10 region of promoter2 was mutated to a constitutive 10 region. nis Z promoter lost function when the spacer of the 10 and 35 regions of promoter2 was reduced from 20bp to 17bp. The results indicate the spacer play an important role on induction and expression of nis Z promoter.