A NEW RESOLUTION VECTOR WITH CRY1ACiO GENE BASED ON BACILL US THURINGIENSIS TRANSPOSON Tn4430
利用苏云金芽胞杆菌转座子Tn4430构建含cry1Ac10基因的解离载体

A new resolution vector with crylAclO gene based on Tnp I-mediated site-spe-cific recombination system of Bacillus thuringiensis (Bt) transposon Tn4430 was developed.The gene crylAclO, encoding a protoxin against plutella acylostella larvae, and the gene oril030, from a plasmid of wide type Bacillus thuringiensis, were inserted into two copy sets of RES sites, named pBMB801. When pBMB801 was introduced into crystal negative Bt host BMB171, antibiotic resistance genes and other non-Bt DNA can be selectively eliminated. This recombinant plasmid was found very stable without antibiotic selection. The resulting strain only contained Bt DNA and is free of antibiotic resistance genes. This strategy should facilitate regulatory approval for its developemnt as a commercial biopesticide.