Accumulation Dynamics and Immuno-gold Location of Movement Protein of GFLV-H in vivo of Chenopodium amaranticolar and C.quinoa
葡萄扇叶病毒移动蛋白在寄主体内的动态检测和免疫金标记

The antiserum of the movement protein of Grapevine fanleaf virus F13 (GFLV-F13) was used to detect corresponding protein accumulation of GFLV Hangzhou isolate (GFLV-H) in systematically infected leave of C.amaranticolor by Western blot analysis. The results showed that the MP was detectable 3 days past of inoculation (dpi), and its amount raised as the time extending, reaching the highest level at 16dpi. Movement protein could be detected stably even though the infected leaves became yellow and drying. The observation of ultra sections of GFLV-H-infected leaves of C.amaranticolor and C.quinoa by transmission electron microscope found that the membrane-walled tubules contained virus particles often aggregated in cytoplasm. Virus particles are aligned in tubular structure in single line, protruding though the cell wall within plasmodesmata in systemically infected C.quinoa. The MP of GFLV-H was located in the cytoplasm near cell wall, cell wall, and plasmodesmata and also on the tubule structure in cytoplams of infected C.quinoa and C.amaranticolor by immuno-gold labeling. So, it could concluded that the movement of GFLV-H between cells is through the tubular structure.