MELANIN PROPERTIES STUDIES OF THE RECOMBINANT OF PSEUDOMONAS PSEUDOALCALIGENES CONTAINING TYROSINASE GENE
类产碱假单胞菌酪氨酸酶基因重组子所产黑色素性质的研究

Pseudomonas pseudoalcaligenes is a notably killing maggots bacterium, which was isolated from natural dead maggots.Tyrosinase gene of P.maltophilia AT18 has been introduced into P.pseudoalcaligenes, and enabled it the ability of producing melanin steadily. Antiradiation effect of the melanin is quite strong. The melanin of the recombinant is nonfixiform material.It's solubility is very little in many sorts of organic or inorgonic solvent. It's solubility is big in alkaline solution, but little in neutral or weak acid solution. It is deposited when pH<4. It is oxidized by H-2O-2 or NaC10. It is also reduced by Ag or H-2S. It has free radical and it can absorbs free radical generated by ultra-violent ray. It can absorbs ray of all sorts of wave length. The absorption rate of ultra-violet ray is the biggest in all sorts of wave length. It can effectively protects protein, DNA and other biomacromolecule matter against the damages by ultra-violet ray.