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微生物学报 1994
STUDY OF THE CHARACTERISTICS OF THE FUSANTS FUSED BY FRANKIA STRAIN Cc01 AND STREPTOMYCES AUREUS STRAIN GL
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Abstract:
Three fusants were obtained by fusing the protoplasts of Frankia strain Cc01 and Streptomyces aureus strain GL. All three fusants grew fast like GL, and could nodulate and fix nitrogen like that of strain CcOl. The colors of the hypha of the three fusants were different from their parent strain GL when they grew in agar media. All three fusants had vesicles which was the typical shape of Frankia strains, and chian spores like strain GL. They all had the antibacterial action to E. coli as their parents. Moreover, the antibiogram of fusant F4 was almost the same as its parent strain GL. Being transfered more than 10 times in the agar medium, all three fusants still had nodulation and nitrogen fixation abilities, but less than their parent Cc01. The serulogical analysis indicated that fusants F1 and F6 had both parents characteristic antigens, while fusant F4 had only the GL characteristic antigens. This suggested that fusants F1 and F6 were more stable in genetics and more believable.