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微生物学报 1996
TRANSFERING OF PLASMID BETWEEN E. COLI AND RHODOBACTER PALUSTRIS
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Abstract:
The broad host range plasmid RP4 and two plasmid gene vecters, pJRD215 and pRK404, were transfered into Rhodobacter palustris strain Y6 by conjugation between E. coli and Rb. palustris. The Km resistance gene of RP4 and pJRD215 and the Tc resistance gene of pRK404 could express in Rb. palustris. The plasmid stability test showed the three plasmids could be maintained stabily in Rb. palustris. This work provide a possibility of the genetic engineering research of Rb. palustris.