Inhibition and Screening of Beneficial Endophytic Bacteria to Control Tobacco Brown Spot
防治烟草赤星病有益内生细菌的筛选及抑菌作用

bacterial strains were isolated from inner of tobacco leaf . It was found that by pairing culture on PDA, 11 potential biocontrol isolates showed good antagonistic effects on 4 strains of different virulent Alternaria alternata, the pathogen of tobacco brown spot, and the inhibition zone diameter ranged from 5 5mm to 13 2mm. The result from experiment trial at greenhouse and pairing culture showed the effectiveness of bacterial preparations from Itb162 on reduction the incidence of tobacco brown spot. The control efficiency reached 52 0%. The results from culture filtrate showed that Itb162 was effective for inhibiting mycelium growth and reducing spore germination of A.alternata in certain of concentration.