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微生物学报 1998
GROWTH OF METHYLOMONAS Z201 CELLS AND PRODUCTION OF EPOXYPROPANE MONOPHASIC AND BIPHASIC FERMENTATION SYSTEMS
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Abstract:
The growth of Methylomonas Z201 cells and production of epoxypropane in money and biphasic fermentahon systems were studied. In monophasic fermentation systems, the inhibitions of propene and epoxypropane on the growth of Methylomonas Z201 cells were observed and the concentration of epoxypropane reached 1.3 mmol /L. In biphasic fermentation systems, hexadecane acted as the "reservoir" of growth substrate (methane) and reactants (propene, molecular oxygen), the decrease of propene and epoxypropane in aqueous phase reduced the ichbihon effect of propene and epoxypropane on the growth of cells, the concentalons of epoxypropane in both wader Phase and hexadecane phase reached l.7mmol / L and 2.6 mmol / L. In both monophasic and biphasic fermentation systems, the operational stability of cells was enhanced compared tO that of reshng cells.