THE IDENTIFICATION OF BmNPV gp64 ENVELOPE GENE
家蚕核型多角体病毒gp64基因的克隆和全序列测定

gp64 glycoprotein, an abundant and the only membrane glycoprotein encoded by the baculovirus genome, contains a signal peptide sequence at N-terminus, a transmembrane anchor at cterminus and a ectodomain between them. The efficient signal seguences and the transmembrane anchor sequences of AcMNPVgp64 gene have been exploited in baculovirus expression system. Using 1.3kb AcMNPV gp64 gene coding sequence as the probe, the nucleotide sequence of BmNPV gp64 gene was determined, and a 1530 nucleotide open reading frame flanked by an AT-rich sequence was identified that could encode a polypeotide with 509 amino acid residues. Computer analysis indicated BmNPV gp64 gene is 84.3 % identical to AcMNPV at the level of nucleotide sequence and 94.7 % identical at the level of predicted amino acid sequence.