CLONING AND ENDONUCLEASE DIGESTION OF lon GENE FROM STREPTOMYCES ANSOCHROMOGENES
圈卷产色链霉菌lon基因的克隆及酶切分析

Streptomyces ansochromogenes is a nikkomycin producer which has typical life cycle of Streptomyces differntiation on glucose asparagine medium. The lon gene product is an ATP-dependent serine proteinase. The lon gene might have some connection with physiological differentiation and also with the morphological differentiation in S. coelicolor. The lon-like gene of S. ansochromogenes was identified by hybridization and the Ion-like gene in 4.6kb PstI fragment was cloned. The physical map of this fragment and the localization of the target gene were preliminarily studied.