FERMENTATION CONDITIONS OF ENGINEERING STRAIN UTILIZING STARCH FOR PRODUCTION OF ALKALINE PROTEINASE
利用淀粉的碱性蛋白酶工程菌的培养条件

The highest activity of alkaline proteinase produced by Bacillus pumilus c 172(pBX 96) transformant was 9 000 U / ml in shaking flask where corn meal and bean cake meal were carbon and nitrogen sources, respectively. The enzyme activity was raised under the conditions of initial pH 7.0, MgCl2 instead of MgSO4, and glucose (0.1%) in the substrate. The fermentation of c172(pBx 96) transformant was carried out with parameters of pH, reducing sugar, total sugar and enzyme activity.