Amplification of Bacterial Heat Shock Protein 60 Gene Using Inverse PCR Method
利用反向PCR方法扩增细菌热激蛋白HSP60基因

A method is presented for rapid in vitro amplification of DNA sequences of bacterial HSP60 gene.In our previous work,using degenerate oligonucleotide primers for conserved regions of HSP60 gene,a 600bp fragment was amplified by PCR,cloned and sequenced.An inverse PCR method,with the primers oriented in the reversed direction of the usual orientation,is used to amplify the DNA sequences that flank the 600bp known region.The feasibility of this method is shown by amplifying the complete HSP60 gene sequences of 4 bacterial strains,namely Bifidobacterium denticolens,Bifidobacterium inopinatum,Bifidobacterium adolescentis and Gardnerella vaginalis.