Identification of Non-essential Region for Fowl Adenovirus Replication of JS Strain
禽腺病毒江苏株（JS）复制非必需片段的确定

The non-essential region of a strain of fowl adenovirus group I virus (FAVI-JS) isolated from chicken was identified. The left terminal (L fragment) and right terminal (r fragment and ITR fragment) of virus genome were respectively amplified by PCR. PCR products were cloned into pGEM-T easy vector respectively. L fragment, ITR and r fragment were then cloned into pHC cosmid to construct pHC-FAVI-r-ITR-L. The enhanced green fluorescence protein (eGFP) coding sequence was cloned between r fragment and ITR fragment to construct transfer vector pFAVI-eGFP. Then the pFAVI-eGFP was transfected CEK infected with wt-FAVI-JS.The recombinant FAVI (rFAVI-eGFP) were recovered by homologous recombination in CEK between wt-FAVI-JS genome and pFAVI-eGFP DNA. rFAVI-eGFP was purified by serial dilution of the supernatant of transfected cells. These results showed that the region between r fragment and ITR fragment was dispensable for virus replication in vitro.