STUDIES ON SUPEROXIDS DISMUTASE OF YEAST
酵母菌中超氧化物歧化酶的研究

A method of breaking cell with toluene has been used for the first timeto extract SOD from yeast, and we have compared this method with other threemethods, we found that SOD activity of crude extraction by this method were respectively 1.29, 1.08 and 2.01 times higher than the methods of treating with chloro-form-ethanol, snailase and cell autolysis. As well as in 5 yeast strains, we discovered that the tolerance of yeast cell to O2 closely related to their content of SOD.The varied culture conditions of one high SOD activity strain (Saccharomyces carl-sbergensis BY2) were studied, the results showed the effects of those conditions onthe content of SOD and SOD isoenzymes.