NUCLEOTIDE SEQUENCE OF THE RHIZOBIUM HUAKUII COMMON NODUTION GENES nodA AND nodBC
紫云英根瘤菌共同结瘤基因nodA和nodBC的核苷酸序列

An another clone pRaN109 which contains nod genes was isolated and identified from the clone bank of R huakuii 159 DNA using the 32P-labeled 2.3kb R meliloti nod DNA fragment as a hybridization probe. Analysis of homologous DNA-DNA hybridization and nucleotide sequence indicated that the 9kb EcoRI fragment of pRaN109 DNA carried the nodD1BC genes and the 18kb EcoRI fragment of pRaN109 DNA contained nodD2A genes. The common nodA gene was separated by 6.7kb from the nodBC genes. Both the nodA and bodB-nodC cistrons showed nod box upstream of these genes. Nucleotide sequence analysis of the genes describes a obviously different organization of common nod genes in R. huakuii 159 compared with that reported for most of the strains from different genera described up to now.