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微生物学报 2004
Identification of A Strain Xenorhabdus sp.with High Antibiotic and Insecticidal Activity
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Abstract:
Strain of Xenorhabdus sp. CB6 with high antibiotic and insecticidal activity was isolated from the intestine of Steinernema carpocapsae collected from orchard of Beijing, China. The morphology, physiological and biochemical characteristics were studied. The results indicated that its characteristics were most consistent with X. nematophila. The sequence of 16S rRNA fragements were analyzed. The homology between strain CB6 and other strains of X. nematophila is above 99%. Strain CB6 was clustered together with other four strains of X. nematophila in phylogenetic tree. But there are some differences in biochemical characteristics (e.g. tyrosinase, lipase, acid from ribose) with other strains of X. nematophlia, especially CB6 has high insecticidal and antibiotic activity. We suggested that strain CB6 was a variation of X. nematophila and named X. nematophila var. pekinegnsis.