STUDIES ON FUSED RECOMBINATION OF GIBBERELLA FUJIKUR OI-GIBBERELLIN-PRODUCING STRAIN
赤霉素产生菌——藤仓赤霉菌融合重组的研究

A pair of nutrient complementary auxotrophic mutants were used as fusion parents. Their protoplasts were prepared enzymatically and PEG4000 was usedas fusion agent. The prototrophic recombinants were selected directly on the minimal regeneration medium. The recombination frequency was about 10-7. Some unstable heterocaryons were occured and the frequency was about 10-5-10-6. Mutation of pigment, hyphae morphology and gibberellin production were induced throughthe fused recombination. The positive mutation, negative mutation of gibberellinyield among the recombinants were 15.3% and 53.1% respectively. Gibberellin yields of recombinants signed RN2 and RG14 were over 25% than that of the prototrophic parent 207 strain.