Preliminary Study on DNA Microarray for Detection of Bacillus anthracis
制备炭疽芽胞杆菌检测基因芯片的初步研究

To develop a technique of detecting Bacillus anthracis with DNA microarray, and explore the way of preparing DNA microarray for detecting B.anthracis. B.anthracis plasmids, pX01 and pX02, were digested with Sau3AI and the resulting fragments were used to construct DNA library of pX01 and pX02 DNA microarray probes were obtained from fragments of DNA library. The positive clone fragments from DNA library verified by sequencing were used as the DNA microarray probes. A plant gene that includes GATC restriction site was used as positive control. When fluorescence labeled, it was added into the sample detecting system. Another plant gene that was nonhomologous to B.anthracis was used as negative control, and DMSO was used as blank control. A DNA microarray of detecting B.anthracis was prepared by printing the probes on a superamine modified glass slide. 290 cloned probes were collected. A DNA microarray for detecting B.anthracis was prepared. After hybridized with sample B.anthracis plasmids, pX01 and pX02, 273 cloned probes were screened by the analysis of the fluorescent intensities for further study. The high effectivity, utility and reliability of DNA microarray technique would lay a good foundation for further study on detection of B.anthracis.