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OALib Journal期刊
ISSN: 2333-9721
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ISOLATION, DETECTION TOXICITY AND STRUCTURE OF TOXIN FROM BEAUVERIA BASSIANA
球孢白僵菌毒素的分离、毒力检测及结构鉴定

Keywords: Beauveria bassiana,Toxin,Spodoptera frugiperda sf-21,cell strain,Heliothis zea,Aedes albopictus
球孢白僵菌
,毒素,草地夜蛾Sf-21细胞株,棉铃虫,白纹伊蚊

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Abstract:

In this experiment, the crude toxin from the metabolite of Beauveria bassiana was isolated and its toxicity was detected. The result shows that indicates toxin has toxicity on larva of Aedesalbopictus obvious. Though it with low toxicity to larva of Heliothis zea when swallowed, but has high toxicity when injected into the blood. For bacteria it only inhibites G . When it applied to cultured cells of Spodoptera frugiperda sf-21, the regression equation of toxicity is y = 2.03 2.39x. The half lethal concentration (LC50) is 0.01751%. 95% believable limit is 0.01517-0.0202%. There are three substances which have similar atomic group when analysed by HPLC and infrared spectrum.

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