Study on Enhances of The Antioxidation in Escherichia coli by Expression of Deinococcus radiodurans pprI
耐辐射球菌pprI在大肠杆菌中表达增强细胞抗氧化能力的研究

The Deinococcus radiodurnas pprI, a switch gene responsible for DNA repair, was transformed to E.coli via a shuttle plasmid pRADZ3 and a recombinant fusion PprI protein was expressed in normal growth condition without induction. The stable expression of PprI protein in E.coli TG1 was confirmed by Western blot. Empty plasmid pRADZ3 was transformed to E.coli TG1 as control. The viabilities under H 2O 2 oxidative stress and the differences in catalases(KatE, KatG) activities of these two reconstructed E.coli TG1 were observed. The results showed that either in exponential phase or stationary phase, the expression of PprI protein in E.coli TG1 can enhance the viabilities, compared with the relative control; The results obtained from the Native-PAGE indicates that the expression of pprI in E.coli TG1 can enhance the enzymatic activity.It is concluded that the expression of D. radiodurans pprI can enhance the antioxidation in E.coli.