PRODUCTION OF MONOCLONAL ANTIBODIES TOBROAD BEAN WILT VIRUS AND APPLICATION IN VIRUS DETECTION
蚕豆萎蔫病毒单克隆抗体制备及检测应用

Six hybridoma cell lines secreting monoclonal antibodies(MAbs) against broad bean wilt virus(BBWV) were produced by fusing mouse myeloma cells(SP 2/0) with spleen cells from BAL B/c immunized by the BBWV particles. The hybridoma cell lines secreted MAbs stably after cultured in vitro for 3 months or stored in liquid nitrogen and then revived for several times. The titres of ascitic fluids of six MAbs ranged from 1:256000 to 1:640000 when measured by indirect ELISA. In agarose gel immunodiffusion test, it showed that the six MAbs represented the same isotype of murine antibodies, IgG1. Six MAbs could detect 4 tested BBWV isolates, but didn't crossreact with other 5 plant viruses. The result of Western blot showed that all the six MAbs can react with the 44 7kD large coat protein subunit of BBWV. This is the first report of production of MAbs against BBWV.