CLONING AND EXPRESSION OF VHB GENE IN D-ARABITOL PRODUCING YEAST
细菌血红蛋白基因在产D-阿拉伯糖醇酵母菌中的克隆与表达

Recombinant plasmid pVgb\|EX2 containing Vitreoscilla hemoglobin gene vgb and formaldehyde resistant gene SFA1 was constructed and transformed into D\|arabitol producing yeast strain Saccharomyces sp.X\|62.The fact that the amount of VHb in transformant cells was considerably higher than that in control cells indicated that gene vgb was expressed in transformant cells.D\|arabitol productivity and yield of fermentation by transformants were improved.The most improvement of D\|arabitol productivity in repeat experiments reached 27.3%.It appeared that the fermentation productivity of D\|arabitol was relative to the amount of VHb in cells under experimental conditions.