A STUDY ON CITRUS TATTER LEAF VIRUS
柑桔碎叶病毒研究

Citrus tatter leaf virus(CTLV)was mechanically transmitted to 23 herbaceousspecies from eight families. It induced local lesion on Vigna sinensis, systemic yellow spotand distortion on Chenopodium quinoa and C. amaranticolor. local spot or Celosia cristataand systemic light mottle on Nicotiana celevilandii. It was also back transmitted fromN. celevilandii to Citrus reficulata,C, citrange and caused typical "tatter leaf "symptoms.DEP for this virus was 10-5, when tested from infected leaf sap of C. reticulata. Virusparticles that characterized as Capiloviruses were detected under electron microscopefrom leaf-sap of diseased hosts above. Herbaceous species can be used as indicators forthis virus. Virus crystallized bodies were found in phloem cells of C. reticulata anc C.citrange infected with CTLV, the same structures were also observed in mesophyll cellsof Chenopodium quinoa and N. clevilandii infected with CTLV. but the crystallizationbodies were looser aggregated and smaller. Degradation of reticulum and accumulating ofstarch bodies was observed on chloroplasts of infected mesophyll cells of above hosts.This is the first report of cytopathological characteristics of this virus disease. Positiveserological relationship was found between CTLV and apple stem grooving virus( ASGV) by agar double diffusion test and ISEM. This indicated the possibility for usingASGV antibodies to detect CTLV in plant materials.