Studies on Hydantoinase Producing Conditions of Arthrobacter K1108
节杆菌K1108乙内酰脲酶产酶条件研究

The hydantoinase-producing conditions from strain Arthrobacter K1108 were investigated.It is shown that the hydantoinase in the strain is intracellular and inducible.Its optimal inducer is 5-benzylhydantoin,while 5-indolylmethylhydantoin and 5-phenylhydantoin cannot induce the production of hydantoinase in K1108.A gratuitous inducer was designed,with which the hydantoinase production is 343% as much as that with 5-benzylhydantoin.The media for culturing the bacteria were screened and optimized.Under optimal conditions,the specific activity of K1108 cells reached 10.8U/mL.