ANALYSIS OF EXOANTIGENS FROM FUSARIUM BY SDS-PAGE AND IMMUNOBLOTTING
镰孢体外抗原的电泳及免疫印渍分析

The partial characterization of exoantigens and mycelia soluble proteins from Fusarium were analyzed by SDS-PAGE and immunoblotting. The results showed that all three exoantigens and three mycelia soluble proteins from F. sporotrichioides, F. poae and F. graminearum have their own patterns differed from each other in gel. Immunoblotting analy- sis demonstrated that the exoantigens were more suitable for being used as an indicator for taxonomy because the specificity of antisera to exoantigens was better than that of antisera to mycelia soluble proteins. The antisera to exoantigens did not cross-react with extracts of grains but tended to have variable degree of cross-reactivities with exoantigens from different Fusarium species. The molecular weight of immunodominant antigens from Fusaria is above 28000.