STUDIES ON THE REGULATION OF CELLULASE SYSTEM BY ATP AND cAMP IN MYCELIAL FUNGI
腺苷三磷酸和环腺苷单磷酸对丝状真菌纤维素酶合成的调节

The intracellular ATP contents in 4 strains of mycelial fungi, grown on the glucose-inorganic salts medium, were determined by luciferin-luciferase system. The results showed that the extracellular cellulase (FPA) of the fungi was synthesized only when the intracellular ATP level was lower than 10-8mg/ml. No matter what carbon source was used, cellulase synthesis was repressed once the intracellular ATP level was over 10-7mg/ml. The cellulase synthesis of the fungi had a negative relativity to their intracellular ATP contents. The cAMP content of the fungi was determined by HPLC. The fungal cellulase synthesis was increased by exogenous cAMP under depression conditions. However, exogenous cAMP could not relax the repression of cellulase synthesis when it had happened. The levels of intracellular ATP and cAMP are the essential factors in the regulation of cellulase synthesis in mycelial fungi.