CHEF GEL ELECTROPHORESIS AS A MEANS FOR PROBE OF CHROMOSOMAL ABERRATION INDUCED BY PROTOPLAST FUSION OF DISTANTLY RELATED BASIDIOMYCETES STRAINS
用CHEF凝胶电泳探测远缘担子菌间原生质体融合后的染色体变异

Two kinds of intergeneric fusants were obtained by protoplast electrofusion between Coprinus cinereus Co5104 (his-) and Pleurotus florida Pf67 (ade-): one is unstable monokaryon which continually segregated when sub-cultivated, the other is stable dinucleic heterokaryon. Comparison of Chromosomal Length Polymorphism (CLP) between fusants and their parental strains was conducted with Contour-clamped Homogeneous Electric Field (CHEF) gel electrophoresis. The result revealed that the deletion of entire chromosomes is the principal chromosomal aberration of fusants. Furthermore, combined with the results of morphological observation and DAPI nucleic fluorescence staining, this result of molecular karyotyping implied that: nucleic fusion, which follows the cytoplasmic fusion, probably takes place in the monokaryotic fusants, and the final genetic composition of this kind of fusants is mainly the chromosomes from parental strain Co5104 as a result of the continual deletion of chromosomes from the other parental strain Pf67. On the other hand, in dinucleic fusants, the nucleic fusion does not occur after cytoplasmic fusion; however, only a proper- tion of chromosomes from each parental stain were retained in this kind of fusants.