Expression of Rice Grassy Stunt Virus in Rice Protoplasts
水稻草矮病毒在水稻原生质体中的表达

With the rice protoplast infection system, the purified virus particles of Rice grassy stunt virus were delivered into rice protoplasts derived from suspension cells of rice cultivars Tainong 67 via PLO-mediated method. Using antisera prepared against the purified virus particles of RGSV, NS3 fusion protein and purified NS6 protein, expressison profiles of RGSV and its encoded proteins in rice protoplasts were investigated by indirect ELISA and Western blot. The results showed as follows: RGSV was detectable in rice protoplasts 24 h after inoculation, and reached its highest level 36 h after inoculation. NS6 was detected 15 h after inoculation, and reached its highest level 30 h after inoculation.