Repetitive-element PCR Genomic Fingerprinting of The Genus Streptomyces
链霉菌的rep-PCR基因指纹分析

Repetitive element PCR (rep PCR) genomic fingerprinting with the BOXA1R primer was investigated as a molecular typing tool for strains of Streptomyces. The results indicated that rep PCR DNA fingerprints of Streptomyces strains were relatively unique, simple, stable, and reproducible. To some extent, rep PCR generated genomic fingerprint analyses yield results that are in agreement with 16S rDNA sequences study. This method appears to reflect the genotypic, phylogenetic and taxonomic relationships of the genus Streptomyces in the species strain level. Therefore, it can be used as a rapid and efficient means of determining taxonomic diversity and phylogenetic structure, especially of large collections of strains.