Cloning of cDNA Encoding Lignin Peroxidase (LipH8) from Phanerochaete chrysosporium and Expression in Pichia methanolica
木质素过氧化物酶基因（LipH8）的克隆及在甲醇毕赤酵母中的表达

The expression of lignin peroxidase gene in Pichia methanolica was investigated. The cDNA encoding lignin peroxidase was cloned by RT_PCR using RNA of P.chrysosporium as template. The lignin peroxidase cDNA was inserted into plasmid pMETA and transformed into Pichia methanolica pMAD16. PCR results indicated that the lignin peroxidase gene from P.chrysosporium has been successfully cloned into Pichia methanolica. The transforments was screened to get high expression strain by shake_flask culture. The extracellular lignin peroxidase activity in this primary construct was 932U/L.