NUCLEOTIDE SEQUENCE ANALYSIS OF THE MINIMAL REPLICON OF THE STREPTOMYCES PLASMID pSGL1
链霉菌质粒pSGL1最小复制子序列测定及分析

The high copy number plasmid pSGL1(7.4kb) was isolated from Streptomyces globisporus . Deletion experiments showed its minimal replicon is located on the 2.0kb Sau 3 AI fragment. This fragment was subcloned. DNA sequence data analysis showed this fragment is a new sequence. Only an open reading frame with high coding probability is located on the minimal replicon. The deduced protein contains motifs characteristic of replicase for rolling circle replication.