Identification and quorum sensing of dominant spoilage bacteria from Litopenaeus vannamei
凡纳滨对虾优势腐败菌鉴定及其群体感应现象

This study was to investigate the dominant spoilage bacteria (DSB) of shrimp and detect the quorum sensing mediated by N?acyl?homoserine lactones (AHLs). The strains of DSB were identified by 16S rRNA gene sequence. AHLs of DSB were detected with strain Chromobacterium violaceum CV026. The results indicated that two strains Aci-1 and Aci-2 belong to Acinetobacter spp.. Both bacterial strains can secret AHLs, and the biofilm formation of Aci-1 were significantly affected by exogenous AHLs. There is a positive relationship between the AHLs activity of DSB and the total plate count and TVB-N content of shrimp, and the correlation coefficient is 0.846 6 (P<0.05) and 0.986 7 (P<0.01), respectively. The DSB of shrimp have the quorum sensing system mediated by AHLs, which plays an important role in the spoilage of shrimp.