Study of Particulate Methane Monooxygenase From M.3011
颗粒性甲烷单加氧酶的研究

Particulate Monooxygenase (pMMO) and NADH-dehydrogenase were purified from membranes of Methylosinus trichosporium IMV 3011. Factors such as Cu 2+, different exogenous electron donors, ethylene diamine tetraacetic acid(EDTA) had effects on the activity of pMMO. Molecular weight of pMMO was determined by HPLC, and content of metal ion from the active center by atomic absorption spectrum analysis. The concentration of Cu 2+ had no effect on the production and activity of pMMO in the bacterial culture, but influenced the stability greatly during the purification of pMMO. Transmission electron microscope (TEM)photos of M. 3011 with different copper concentration showed that the concentration of copper decided the quantity of pMMO. Quinol was an effective exogenous electron donor for the purfied pMMO, whereas NADH aws ineffective, which meant NADH-dehydrogenase was unnecessary to be co-purified. It facilitated further study on the catalytic core of pMMO.